i have lyophilized specific antibody to dehydrin "group close T " and i need solve it what buffer can i use to solve ?
It depends on your further use of this antibody and on the approximate isoelectric point of the antibody. If you are going to use it for any binding assay you can probably try dissolve it in some buffer with pH around 7.0 - 7.5 and NaCl concentration about 150 mM. If this is close to the isoelectric point of this antibody or the antibody is insoluble at this pH you need to use pH at least 1.0-1.5 units up or down of this point. If you are going to run some assays to characterize this antibody on different parameters besides functional properties (MW, physical integrity, isoelectric point, amino acid sequence, amino acid analysis, isotyping, carbohydrate composition, or any other physico-chemical characterization) you probably need to dissolve it in some low salt buffer like 10 mM of Phosphate Buffer with pH about 7.0-8.0 (depending on solubility). But each antibody requires its own optimal buffer (should be tested in each experiment).
Thank you for clarifying Dear Viktor Y Butnev i well use in in western blotting
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