cosmarium moniliforme

de todas formas rectifica, creo que es esa

please go for DNA Identification at NCIM, Pune , India.it is a mixed  algae species.The tiny green ones look like scenedesmus sp.THE other brown chain ,you better send your samples  for proper identification by NCIM, PUNE, here at India.

Dear Anil:

In my opinion, you have a member of the former chains of Gymnodiniium (Gymnodinium and Cochlodinium), a chain in the first image, a division cell or fusion of gametes in the second, and a cyst in the third. You must look in this Order to determine this material, you would need better images in different foci showing cingulum and sulcus.

Cosmarium belongs to the Order Zygnematales, Family Desmidiaceae. They are Chlorophyta, their color is green, and this group is restricted to fresh water. Aditionally the core of its material is characteristic of a Dinophyta (a mesocarion), different from the core of Chlorophyta.

It is perfectly possible to determine the phytoplankton based on morphological analysis, it is not necessary to use molecular methods to identify the material in the samples.

I recommend chapter 2 Dinoflagellate of Steininger & Tangen included in Identify phytoplanckton Tomas (1997) and a general text to read about Dinophyta as Lee (2008).

Best regards.

Eugenia

It can be doliole in the first image

I agree with Eugenia, it is likely a dinoflagellate. Certainly the last image shows a large nucleus (dinokaryon) and an inkling of a cingulum. It doesn't look like it contains chlorophyll, you might want to check it alive or preserved with glutaraldehyde. For LM identification however it looks like a hopeless case...

best wishes, René

This is an unarmored marine dinoflagellate. The chains are common in Gymnodinium catenatum and allied species, or Cochlodinium polykrikoides and allied species. If the picture 1 is upside down, the nuclei are located in an anterior position, as usual in Cochlodinium polykrikoides or allied species. It is hardly visible, but there is a kind of red or orange organelle that may correspond to eyespot or stigma. This is a diagnostic feature in Cochlodinium polykrikoides and allied species. As the cells are close to lysis, they are deformed and it is difficult to observe other diagnostic characters such as the position of the sulcus and cingulum.

Your cells may correspond to the harmful species Cochlodinium polykrikoides or C. fulvescens.

Respected Eugenia A Sar, Rene Van Wesel, Fernando Gomez and all others thank you for your valuable answers and suggestions. I searched in websites and found cochlodinium sp. shows similar kind of cell structure. Once again Thank you all.

Anil P

If you enlarge the first picture to about 400, you see at the upper 3 cells clearly the characteristic transverse flagellum of a dinoflagellate, therefore I agree with Eugenia and Fernando, that it might be a colony-forming species of Gymnodinium, Cochlodinium or related genera. In contrast to René, I am the opinion, that it is a photosynthetic species, but agree with him that species and even genus identification is not possible from these picture.  

Cochlodinium sp. I agree Mr. Gomez, those are unarmored marine dinoflagellate...

All best