I want to isolate mitochondia from liver tissue in wistar albino rats.
Google is your friend, online there are lot of articles, this one is from nature protocols (http://www.nature.com/nprot/journal/v2/n2/full/nprot.2006.478.html)
Check another one from experimental biosciences. (http://www.ruf.rice.edu/~bioslabs/studies/mitochondria/mitoprep.html)
If you can afford kit for isolation of mitochondria, here it is.
Mitochondria Isolation Kit for Tissue (ab110168) (http://www.abcam.com/mitochondria-isolation-kit-for-tissue-ab110168.html)
Well, we have used a commercial kit available from Biovision Labs USA. The product is economical and has a user-friendly protocol. We have a couple of publications in which this particular kit was used. Please refer to the kit Technical Data Sheet and follow the instructions for tissue samples.
Article Occult hepatitis C virus elicits mitochondrial oxidative str...
Data Role and clinical significance of lymphocyte mitochondrial d...
The above answers are very adequate. We isolate mitochondria on a routine basis from many tissues including liver. But the key questions are: what do you want the mitochondria for and how pure do you need them.
thanks for your contribution , actually virgilio sir, i want to isolate mitochondria by normal manual method , because we cant afford kit for that and check enzyme assay of electron transport cycle .
Arbind,
This paper provides you with the protocol for the isolation method for several tissues, including the liver and it was the basis of the protocol in our lab.
Hope this helps and good luck.
please refer enclosed paper
Article Association of mitochondrial nitric oxide synthase activity ...
Mitochondria Isolation Kit (Miltenyi)
In case you are interested in the mitos of only one cell type it might be a good idea to first perform a cell isolation step using MicroBeads.
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