What is the best technique to identify native bacteria groups present in soil?

I am lay in this subject. Is DNA sequencing the best way to identify the different types of soil bacteria present in soil samples? Ideally, I'd like to find a technique that allows me to identify...

29 September 2020 4,609 9 View

Do you have any suggestion about constrained estimation of mixed models using lme4 package of R?

I would like to use the lmer function of the lme4 package to estimate the random part of the model given the coefficients of the fixed part (which have been estimated in a previous stage using...

24 February 2020 1,731 5 View

Can I use Nanostring microRNA count date (normalized or raw data) to measure relative gene expression?

Can I use Nanostring microRNA count data (normalized or raw data) to measure relative gene expression by comparing it with an endogenous control (mRNA) expression?

25 November 2019 277 2 View

ERT Optimisation protocol sequence software-code for 3D unconventional electrode arrays?

Anyone know about any freely available software or code that can optimise protocol sequences for 3D unconventional electrodes setups, including borehole electrodes? The closest research I found...

15 November 2019 7,802 3 View

How can I calculate the concentration of 30nM miRNA from a 5nMol stock?

I have a transfection experiment to be done. The stock miRNA is 5nMol. The manufacturer recommends adding 75uL of sterile water, giving me a concentration of 66.67 uM. How can I have a final...

05 May 2019 4,141 7 View

Do we have cell culture contamination?

We are experienced lately some problems with primary cells. We could see (even in 10x magnification) tiny bright particles around the addhered cells. There is no macroscopic sign of...

14 December 2016 7,464 11 View

Could anyone help me with PCR to Mycoplasma detection?

I'm tryint to standardize a PCR to mycoplasma detection of dna from cell culture. I'm testing samples positive for mycoplasma and negative as controls. I found a weak band unspecific slightly...

29 June 2016 7,818 3 View

Low caspase activity in heavy metal lethal concentration 50 (LC50). What does that mean?

02 June 2016 1,480 3 View

How do I read the results of an ANOVA (2 independent factors) that instead of F reason, I got a Z? What is this Z?

I ran a 2 independent factors ANOVA in SPSS and got a Z score instead of F. Is that related to different sample sizes in different conditions? Do I need to even the sizes of the samples before...

08 March 2016 3,813 7 View

Could someone advise me about the best proportion of Ficoll-Paque Premium (1.077g) and bone marrow for isolation of mononuclear cells?

Hi, everyone. I want to isolate mononuclear cells from bone marrow using gradient centrifugation with Ficoll-Paque Premium 1.077g. However, GE's protocol suggests almost 1:1 of ficoll and bone...

21 October 2015 1,694 4 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

How to resolve Gaussian error termination " Error termination via Lnk1e in F:\G16W\l103.exe" for TS(QST3)?

When I run the job for TS(QST3) in gaseous phase, it was completed without any error. But when I add water in CPCM model it terminated with an error: Inconsistency: ModMin= 2 Eigenvalue=...

21 February 2021 3,198 2 View

Using the Pfaffl method: Is it possible that the ratio of the calibrator does not equal 1?

Hi all, I have a question regarding calculations using the Pfaffl method. I have 3 groups per experiment: 1 control group (calibrator) and 2 treatment groups with 6 to 7 biological repeats per...

19 February 2021 8,351 2 View

Phosphate buffer with different pH conditions: HCl or NaH2PO4?

Hi all, I just encountered this dogma; when adjusting pH of phosphate buffers, I've been using HCl/NaOH to in/decrease pH of buffers and recently I was told that HCl/NaOH can't be used to adjust...

08 February 2021 9,070 5 View