Can anyone help me how to analyze (3aR,5R,6aS)-5-hydroxy-2-oxohexahydro-2H-cyclopenta[b]furan-4-carboxylic acid racemate using chiral HPLC method?

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Hao-ran Liu

You can make a try with chiral column manufactured by DAICEL (Janpan) INVESTMENT CO.,LTD.

Mark Robert Prestly

The attached patent may be of interest to you. Various columns and eluents are used including Chiralpak AD with Methanol/Acetonitrile (25:75). However please note that this is for the analogous compound with the acid functionality reduced to the primary alcohol (3aR,5R,6aS)-5-hydroxy-4-(hydroxymethyl)hexahydro-2H-cyclopenta[b]furan-2-one) so you may need change the conditions slightly.

Balaji Babu

Dear Mark,

Thanks for the reference.

Balaji