I need to clone something is SapI site (which is kind of an odd place to clone any recombinant gene) and it's too close (150 bp downstream) to the replication ori (pBR322 based) of the pCA24N plasmid. I am concerned I might disrupt any replication protein around the ori and was wondering if someone knew the replication proteins for this plasmid.?
Article Complete set of ORF clones of Escherichia coli ASKA library ...