Some bacteria, that does not form very good bio-film like A. baumanii, forms only air-liquid interface. It does not form bio-film at the bottom of the plates. Can anyone help me with this issue?
You can use a membrane on a plate filled with liquid medium as in :
Appl Environ Microbiol. 2003 Jul;69(7):3719-27.
Biofilm-specific cross-species induction of antimicrobial compounds in bacilli.
Yan L, Boyd KG, Adams DR, Burgess JG.
it work well I already use it
or a roller bottle (I never tested it) as in :
MARINE BIOTECHNOLOGY
Volume 4, Number 4 (2002), 356-366, DOI: 10.1007/s10126-002-0041-x
Surface Attachment Induced Production of Antimicrobial Compounds by Marine Epiphytic Bacteria Using Modified Roller Bottle Cultivation
Liming Yan, Kenneth G. BoydetJ. Grant Burgess
good luck
Hi Karthikeyan.
The tips from Kientz are very good.
This surface biofilm is directly related to use necessity of oxygen of the strain and varies on each strain (not only in the specie). Some people use a bit KNO3 to help in the O2 diffusion. I never tested it. Some other strains like 1% glucose.
Good Luck and let us know about your attempts.
Alexandre
You could try varying the growth conditions, such as adding different carbon sources to the medium or the aeration.
Thanks Betty and Macedo, I have used extracellular proteins as substrate and also I tried 2% glucose (adding 2% inhibit the growth, may be i need to try 1% and most media has glucose already in the media)
In some bacteria (eg Streptococcus) biofilm production is controlled via the saccharose metabolic pathway, so that adding saccharose to the media enhances biofilm production.
Using subinhibitory doses of antibiotics is possible induce bacterial
biofilm formation. But is very important use several controls if you are developing a research about new products with antibiofilm activity
http://mbio.asm.org/content/3/4/e00198-12.full.pdf
http://dentalschool.umdnj.edu/research/center-files/documents/Kaplan2011_000.pdf
I have very Interesting experience in terms of Biofilm formation by S. aureus. Under the stress partially damaging cell wall e.g. vancomycin, ampicillin the subject isolate showed increased biofilm formation. for complete discussion you may refer manuscript: Mirani ZA, Khan MN, Aziz M, Asadullah, Naz S, Khan SI (2012) Effect of stress on biofilm formation by icaA positive and negative strains of methicillin resistant Staphylococcus aureus. Journal of the College of Physicians and Surgeons--Pakistan : JCPSP [2012, 22(1):10-14]
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