12 Questions 27 Answers 0 Followers
Questions related from Karthikeyan Ramalingam
I am using 5% Polypropylene glycol and 5%Tween 20 in my formulation solution for antimicrobial assay. I want striize Polypropylene glycol and Tween 20 for microbial assay, if I autoclave what are...
29 July 2013 5,469 2 View
I would like to know why isn't the MIC-Minimum Inhibitory Concentration measured as MIC 50 or MIC 90, instead of just 99.9% MIC or complete inhibition? Is MIC 50 acceptable for new antimicrobial?...
29 July 2013 982 5 View
For the new antimicrobial development… As per standard methods, what is the inoculum size one needs to use, in terms of optical density or CFU? What percentage of inoculum does one need to use...
05 June 2013 2,725 2 View
Anybody know how to preserve or store bacterial biofilms for the experiments? Most of the biofilm assays need a longer time to develop biofilms. For this problem, I like to develop biofilms in...
05 December 2012 3,126 6 View
For one antimicrobial the zeta potential is -64mV and for another is 0mV. Is there any relationship between antimicrobial activity and Zeta Potential? Or is it only related to stability of solution?
19 November 2012 480 1 View
I would like help with reproducibility in biofilm quantity and less time consuming (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas...
14 November 2012 2,179 6 View
The biofilm samples will be first dried, resuspended in 1 N NaOH, and heated at 80°C for 30 min in a water bath. The samples will be then centrifuged at 10,000 rpm and 4°C for 15 min. The...
06 November 2012 3,356 4 View
For developing good portable sterilizer for field use (maybe for forest, desert) which source can work with smaller battery source, with less energy, in a smaller box without producing hazards to...
06 November 2012 1,124 2 View
For anti-caries, oral antimicrobial agents and oral health care products development, I am looking for an in vitro mouth model system for experiments. One of my mentors (Dr. Bennett T Amaechi,...
24 October 2012 7,858 2 View
I am working on antimicrobial activity of nanoparticles, for targetted antimicrobial therapy I am looking some help on antibody tagged nanoparticle preparation.
22 October 2012 2,508 5 View
Some bacteria, that does not form very good bio-film like A. baumanii, forms only air-liquid interface. It does not form bio-film at the bottom of the plates. Can anyone help me with this issue?
18 October 2012 745 9 View
I am looking for a method other than HPLC purification.
20 September 2012 9,163 11 View
