I downloaded the trial but I don't really know how it works, I have done some analysis with FlowJo but these two programs are really different.
ModFit is not an user friendly sw. Basically open your sample, choose what channel (parameter) you want to use for cell cycle analysis, define first on your FSC/SSC histogram population you want to analyze, second gating should be done to select single nuclei/cells (PI-H vs. PI-A). Now you should see the familiar cell cycle profile. Than select regions for G1 and G2/M and click on "analysis". (I hope I remember names of these buttons correctly). It is useful to log in as an administrator (psswd is either Omega or omega) to have access to all settings. I don't like their auto analysis modes and wizards. Good luck!
Hello Vania,
I can help you with using FlowJo to analyze your dataset, let me know what particular questions you might or email us at [email protected] for more help.
Best,
Miguel Velazquez
FlowJo Technical Support
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