How much voltage/current should I set to transfer minigel in Western blot?

11 December 2015 9,742 5 View

How can I find a point on a trend line in Excel chart?

I have some data that I need to find 50% value for. The chart consists of the period (days) as X axis values and percentages as Y axis values. I was trying to find a way to determine the 50% value...

06 July 2015 1,228 9 View

Is there any specific procedure to purify a plasmid from Agrobaterium?

31 December 2014 2,724 9 View

Where do turnip aphids exist?

Hi, I wonder if there is anybody who could help with distribution and identification of turnip aphids. What is the most popular ID key used to identify this species? Where have turnip aphids been...

11 December 2014 4,569 6 View

Does the kind of tube used in diluting ELISA solutions affect the test?

Is there any difference between using an eppindorf plastic tube and using a glass tube for diluting the antibodies and the conjugate that are using in ELISA test? I have heard that if I use a...

07 August 2014 3,071 11 View

How can I store aphid samples?

06 July 2014 817 13 View

Do you think that RG should be used as one of the scientific institutions' criteria?

Hi, I have heard that some universities started using RG score as one of the criteria that evaluate the excellence of their faculty members. They are thinking about making the RG score in their...

04 May 2014 8,497 10 View

As a researcher, do you spend more time in your lab or office than you are supposed to?

Some researchers stay at their labs until midnight or maybe later. Some of these people enforce their students to do so, but I see that none of them produce more than other people who leave their...

04 May 2014 8,160 7 View

What are the Pros and Cons of publishing in a high impact factor journal?

Can anybody suggest a scientific journal (or journals) in Biology or Agriculture accepting review articles without invitation (article submitted directly and reviewed)? I am looking for a free of...

04 May 2014 6,837 20 View

How can I separate agarose gel electrophoresis close band sizes?

I have a clone cut by an enzyme and the result of that cut is two bands, which are 12000 bp and 8000 bp. On 1% agarose I was not able to isolate DNA from gel because they appear at the same site...

03 April 2014 5,082 24 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View