Mustafa A Adhab

19 Questions 155 Answers 0 Followers

Questions related from Mustafa A Adhab

Can anyone help identifying the causal agent?

I observed the attached symptoms on Eucalyptus. Do you have any idea what causes these symptoms? When I tried to remove it, in was hard to be removed. It looks like tumors. Thanks in...

02 February 2020 1,531 11 View

How much voltage/current should I set to transfer minigel in Western blot?

Is there any scale for setting voltage/current in running Western blot transfer? Is it different when using different sizes of gel?

12 December 2015 9,837 5 View

How can I find a point on a trend line in Excel chart?

I have some data that I need to find 50% value for. The chart consists of the period (days) as X axis values and percentages as Y axis values. I was trying to find a way to determine the 50% value...

07 July 2015 1,344 9 View

Is there any specific procedure to purify a plasmid from Agrobaterium?

I am trying to purify a plasmid from agrobacterium using Alkaline Lysis method, but it seems very difficult to get it done. Has anybody tried to purify any plasmid from Agro? How? What is the best...

01 January 2015 2,821 9 View

Where do turnip aphids exist?

Hi, I wonder if there is anybody who could help with distribution and identification of turnip aphids. What is the most popular ID key used to identify this species? Where have turnip aphids been...

12 December 2014 4,689 6 View

Does the kind of tube used in diluting ELISA solutions affect the test?

Is there any difference between using an eppindorf plastic tube and using a glass tube for diluting the antibodies and the conjugate that are using in ELISA test? I have heard that if I use a...

08 August 2014 3,164 11 View

How can I store aphid samples?

I want to save some turnip aphid samples temporarily, so I can send them for identification later. I want to know if I can use ethanol and at what percent and is there any other special way to...

07 July 2014 907 13 View

Do you think that RG should be used as one of the scientific institutions' criteria?

Hi, I have heard that some universities started using RG score as one of the criteria that evaluate the excellence of their faculty members. They are thinking about making the RG score in their...

05 May 2014 8,587 10 View

What are the Pros and Cons of publishing in a high impact factor journal?

Can anybody suggest a scientific journal (or journals) in Biology or Agriculture accepting review articles without invitation (article submitted directly and reviewed)? I am looking for a free of...

05 May 2014 6,930 20 View

As a researcher, do you spend more time in your lab or office than you are supposed to?

Some researchers stay at their labs until midnight or maybe later. Some of these people enforce their students to do so, but I see that none of them produce more than other people who leave their...

05 May 2014 8,253 7 View

How can I separate agarose gel electrophoresis close band sizes?

I have a clone cut by an enzyme and the result of that cut is two bands, which are 12000 bp and 8000 bp. On 1% agarose I was not able to isolate DNA from gel because they appear at the same site...

04 April 2014 5,219 24 View

Can anyone help troubleshoot why I got unexpected results from my clone?

I was trying to make a full-length clone of a circular double-stranded DNA virus genome. The genome is 7,753bp in length. A Rolling Circle Amplification (RCA) was applied with a commercial RCA kit...

04 April 2014 9,699 1 View

What is the best way to clone an 8000 bp insert into a 4000 bp vector?

I have experienced some difficulty in cloning one of my inserts. I could get this insert into 2000 bp vector, but I've tried several times to get it into 4000 bp vector, for different experiment...

04 April 2014 8,320 20 View

Will Google Scholar find articles published on Research Gate?

I wonder if Google Scholar can recognize papers on RG and count citation accordingly. Anyone has any thoughts about such cases? I mean an article published only on Research Gate and Google...

01 January 1970 4,746 19 View

Why don't I get positive reaction when amplifying resistance gene in tomato?

While detecting the Mi-9 gene that is responsible for providing resistance to nematodes in tomato, we used C8B primer sets to amplify the genes. after crossing several parents that carry the gene...

01 January 1970 1,781 8 View

What causes the abnormal leafy ears in corn?

What causes the abnormal leafy ears in corn? We have seen this abnormal ears at the end of the season in corn. Usually, at the edges of the field. Not sure about the center of the field (we have...

01 January 1970 6,115 9 View

What causes cucumber wilt in greenhouse?

We have a cucumber wilt that is spreading in greenhouses. I have done some search online and found similar cases in the area (I posted the photos that is similar to the case we have). Please...

01 January 1970 6,068 9 View

Is there a commercial kit to extract fungal DNA from an infected plant tissue?

If I have a plant leaf with Alternaria sp. infection. Is there a commercial kit to extract the fungal DNA only? OR the plant DNA ?

01 January 1970 1,505 11 View

Is Coronavirus really dangerous?

How many people have died because of the Coronavirus infection compared to people who got infected? Is not it real that seasonal flu kills more people every year than Coronavirus does? How is it...

01 January 1970 3,272 34 View