What corporate social responsibilities do social media companies have in relation to the use of psychographic profiling in advertising ?

What are the responsibilities of CSR ? What social media companies have done to protect the privacy or information of their users ?

12 October 2020 433 5 View

Tissue Digestion with proteinase k gives "invisible" globule"?

I'm digesting a few different mammalian tissues with a spin kit and proteinase k. Some tissues (esp. testis and pituitary gland) look fully digested and clear. But once the lysis buffer is added,...

09 August 2020 4,443 2 View

Can I use a spin column kit to extract DNA from bull testis?

I'm extracting DNA from various bull tissues and there are some that don't work with a spin column kit, like brain tissue that contains a lot of lipids. For those, we do Phenol-chloroform...

05 August 2020 2,126 2 View

In what cases does blood pass through implants according to the Darcy law?

Hello! I would like to understand in what cases blood or some other biological liquid passes through implant or graft with some porous microstructure according the Darcy law (thanks to the...

26 March 2020 8,670 14 View

K10/K6 and PHQ9 comparison?

Hi Is anyone aware of a publication or article discussing the convergent validity or crosswalk of the K10 and PHQ9 for a measure of depression? Many thanks

03 March 2020 8,601 1 View

Whats the difference between chitosan -tpp and sodium alginat-cacl2 in the making of nanoparticle?

why most people use chitosan tpp instead of alginat-cacl2 as a nanoencapsulation?

13 February 2020 251 3 View

How to measure adolescents' career maturity / vocational training maturity?

I found literature of and about J. O. Crites' Career Maturity Inventory. I cannot find the inventory tool /items. May someone shares their experiences and ideas on this topic with me? Which...

11 February 2020 1,387 3 View

HEK 293 MSR cells die/detach after transfection?

We have used TransIT-X2 (Mirus) for years to transfect different cell lines, but suddenly I am having a lot of trouble with my HEK 293 MSR cells becoming very round and dying/detaching soon after...

07 February 2020 6,375 3 View

Is it really necessary the clean-up step after PCR for digesting with DpnI?

I've read different protocols, starting for the QuikChange one, and there's no call for cleaning up after PCR. However, I did clean up as suggested by other researchers, so the polymerase doesn't...

12 November 2019 9,677 5 View

Should postgraduate students be required to transcribe research interviews or can they be allowed to analyse the aural (recorded) data?

Should postgraduate students be required to transcribe research interviews or can they be allowed to analyse their aural (recorded) data? I have a postgraduate student who wants to analyse her...

24 October 2019 2,006 6 View

How to simulate 5G Quality of Service characteristics?

For my research, I would like to simulate an end to end 5G network. My goal is to observe the behaviour of SDAP protocol, dynamic configuration of 5QI values etc. To summarize I would like to...

21 February 2021 7,624 3 View

Why the battery connected on the load side for storing purpose is taking the volatge /current at whatever rating it is given in matlab simulation?

I am designing a DAB DC DC Converter and when I am connecting a battery block in Matlab and fix its specification lets say 350 V and 25 A, when I am giving input as 600V, Battery would charge to...

11 February 2021 3,110 1 View

How can we simulate vehicle to infrastructure communication in NS-3?

I am working on handoff management in IEEE 802.11p/WAVE based VANETs. I want to know if I can simulate V2I communication mode using NS-3, which will lead to the simulation of handoff process. Most...

16 January 2021 1,733 4 View

I need to know about immunohistochemistry steps?

Immunohistochemistry staining. Tissue Fixation during IHC. Using DAB. blocking step. controls group.

23 December 2020 5,768 2 View

Which Open Source emulator is best for SDN based IoT implementation?

We would like to implement SDN based IoT secure application. Please suggest best open source Emulator / Simulator.

15 December 2020 8,329 5 View

IHC-P staining of the viral infection in the mouse pancreas?

Hi, I'm trying to stain a viral infection of the pancreas in paraffin sections of adult (3 month old) BALB/c mice. Prior to starting my histology analysis, I have titrated the organ to confirm...

10 December 2020 2,198 3 View

DNA Extraction - best laboratory location?

Hello, we are in beginning NGS projects with rhizosphere soil using Qiagen's powersoil extraction kit. In past labs, I have performed DNA extraction in several locations: chemical fume hoods,...

07 December 2020 9,492 3 View

Luciferase assay for gene-miRNA interaction: which vectors should be used?

23 November 2020 2,813 2 View

What is a recommended method for simulating paste/slurry (Solid-Liquid) 3D dispensing?

The paste is designed to be dispensed from a syringe with a small nozzle. The paste is composed of highly viscous fluidic phase, and several types of solid spheres with different densities. The...

05 November 2020 2,927 2 View

Luciferase assay for miRNA-gene: How to construct the mutated prime?

I have experimental evidence (qRT-PCR and WB) to suggest a miRNA downregulates a gene. To test if the regulation is direct I want to perform a luciferase reporter assay. So I designed a primer...

15 October 2020 7,641 4 View