Can anyone help determine the cause of strange viscosity following ligation of linearized vector with annealed oligos?

11 November 2013 0 6K Report

I'm conducting a protocol where I anneal a set of oligos and then ligate this oligo cassette with a linearized vector using T4 DNA ligase enzyme and buffer overnight at 16 degrees. The overnight ligation reaction looks extremely viscous and sticky--kinda like undissolved DNA. I am not sure what this is and the ligation has not been working. The subsequent steps require bead binding and hence the high viscosity of the ligation is a concern. Has anybody else had this experience? What can cause this sticky, viscosity of the ligation reaction? All oligos are dissolved in nuclease free water.

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