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They are software's that are used mainly in the discipline of psychology

05 August 2020 9,423 3 View

Which value of volume fraction I can to choose for to identify free surface in an air-water simulation of open channel?

In air-water multiphase flow CFD simulation's it is common to use a VOF = 0.5 to track or identify the position of interface or free surface. I would like to know why this criteria is used and if...

12 July 2020 7,793 9 View

How to decide if to get 1 or various P value? regarding an composting process through time and treatments?

I have a research investigation that I got the results, I applied 4 different composting processes to vegetable waste (manure, manure+Effective Microorganisms, Effective Microorganisms and a...

14 June 2020 2,373 2 View

What is a CFD and other question?

To me, a CFD is solve a fluid mechanics problem using mathematical approaches with the help of a computer. In other words, it is like a marriage between a mathematical model and numerical methods...

04 May 2020 2,811 25 View

Purification of histag protein using HISTRAP HP (GE). What is the best lysis buffer and which protocol may be most suitable?

Purification of histag protein using HISTRAP HP (GE). I am working with insect cells (Tn5B) and my antigens are expressed in the nucleus. I am having difficulty lysing the cells because we have no...

10 March 2020 8,609 2 View

Recent meta-analysis on imaging treatment response predictors in depression?

Dear Experts, Would anyone know of any recent meta-analysis of neuroimaging predictors of treatment response in major depression? I have looked around and all I could find was a paper from 8...

27 January 2020 9,717 1 View

Any suggestion for the amplification of Wingless in old collection samples?

Hello to everyone, I'm trying to amplify nuclear markers from old museum specimens of beetle (not very old, sampled 10-15 years ago) , I thought on the Wingless fragment, it's obviously a hard...

02 December 2019 7,463 3 View

How to reduce massive protein degradation during purification?

Dear all, I am expressing in BL21(DE3) E. coli cells and subsequently purifying a 6x-His-tagged 50 kDa truncated protein which looks like to be very unstable. Its domains are literally falling...

17 October 2019 1,416 13 View

Why p-values are higher when I run a logistic regression with all variables together, but significant when I do separately?

I ran an experiment with a 2x2 design, like the dependent variable I have a dichotomic variable. When I running a factorial ANOVA, interaction is significant and one principal effect, also when I...

14 October 2019 9,342 6 View

How to calculate the deltaG reaction in solution from the corrected energies by PCM and SMD?

Hello everyone! I am studying a complexation reaction with a bidentate ligand the type: Ligand + [Zn(H2O)4]2+ --> [Zn(Ligand(H2O)2]2+ + 2H3O+ The geometries of all reaction...

27 August 2019 6,584 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View