Leonardo Campos Zanelatto

4 Questions 4 Answers 0 Followers

Questions related from Leonardo Campos Zanelatto

Anybody could please share a detailed protocol for immunofluorescence?

I never worked with immunofluorescence (IF) before, and I need to do IF in blood cells to detect a specific DNA-RNA hybrid in nucleus. I tried to look for a protocol in the internet and in papers,...

01 November 2016 7,068 3 View

Anyone already ran a agarose gel with cDNA and found no bands or smear? (But in qPCR with housekeeping gene you have amplification and single melting?

Guys,I made a cDNA with High capacity kit and ran an agarose gel (1%, 1ul of cDNA) to check, BUT...there was nothing!!! I didn't had a single band, and not a smear either! Anyone have any idea...

12 August 2016 3,135 4 View

Is there a specific lineage of mouse to make MEFs?

I am looking to produce MEFs (mouse embryonic fibroblasts) and I wonder if the strain of mice have some influence (needs to be a specific lineage) or if I can use any (BALB-C, NOD-SCID).

02 July 2014 9,285 4 View

Can anyone help about the dose of irradiation to inactivate MEFs?

I'm going to inactivate MEFs by irradiation to use it later as feeder cells in iPs. Anyone know the dose (in grays (Gy)) and the time of irradiation that I need to use to inactivate it? I will...

07 March 2014 1,979 3 View