I wanted to check the expression pattern of some abiotic stress tolerance genes for rice. So I wanted to design primers of those genes for real time PCR.
Hi, here a some links:
http://openwetware.org/wiki/Choosing_primers_for_qPCR
http://bitesizebio.com/articles/a-step-by-step-guide-to-designing-qpcr-primers/
http://www.genomics.agilent.com/article.jsp?pageId=2191&_requestid=957449
This book is helpful: Real-time PCR. Taylor & Francis. 2006.
Cheers, Nadine
Dear Madam
Thank you for your answer. After studying the links I will contact you again if there is any query.
Regards
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