When I did MIC for some chemical compounds against MRSA according NCCLS I got difficulty in detection of MIC because there is a turbidity. What is your suggestion? Also I used different media TSB, MHB, cation MHB and MHB with tween 20.
First you need to find the reason for turbidity. If your test compound is not soluble in water, it may get precipitated, giving rise to turbidity. If the turbidity is due to coloured nature of your test compound, then you need to include a proper abiotic control in your experiment.
first of all this compounds bacteriostatic , secondly some compounds of them give clear inhibition against some bacteria , finally i included in my experiment a control drug which give a good inhibition as when compare the activity the control there is slighlty turbidity.
If you used novel compounds, you had be better to include only compound (compound + media) as control. Some compounds may react with media during incubation.
I can give you some suggestion regarding the turbidity problem. I too have worked with MRSA (most unstable organism to work in vitro, though thesse may create a havoc in nosocomial infections). However, I need to know what is the chemical your are working with and causing you problems of turbidity with MRSA. Nevertheless, You can try a lesser accurate method lof plate culture and go by the number of colonies. Your will get a' fair enough' good result.
You can develop E test or use two fold serial dilution, that will lower down the turbidity level.But I advice you to analyze your samples by taking optical.density of the treatments and control samples, you can minus turbidity index obtained test samples from control. You can go for well culture methods and utilize minimum amount of compound and 7 times lesser no. of bacterial colonies in suspension culture.