Climate change and ethics -what could be the participation of citizens in the making of a city in fullfilling the said goal

09 September 2016 1,720 8 View

What is the Role of craft in the building industry?

01 March 2016 9,366 2 View

Coating glass slide with Gold nanoparticles: How to stably bind it and still allow conjugation with other bio-molecules?

im recently very new to experimenting with nanoparticles and i would like to coat gold nanoparticle onto glass slide as well. I thought APS(amino silane) coated slides with positive charge would...

26 January 2015 6,027 8 View

Can normal mode analysis differentiate ligand bound and unbound states?

Can anyone suggest normal mode analysis tools that can be used to differentiate 2 states of the protein- in the presence and absence of a ligand?

06 November 2014 4,730 5 View

Is it possible to find out the occupancy of water within a protein using gromacs?

I would like to plot no. of water molecules within a cut-off distance as a function of time. Any gromacs utility that will help me? Thanks in adv

03 September 2014 685 5 View

Is it necessary to store pH 6.8 and pH 8.8 tris buffer at 4 degrees?

We use to prepare 1.5M tris pH 8.8 and 1 Mtris ph 6.8 for SDS and we keep it at room temperature. After few days I could see there is a increase in pH

24 July 2014 6,288 12 View

I am doing western blotting and the transfer of protein from gel to PVDF membrane is not that muck efficient. How can I trouble shoot it?

Nearlly 5 to 6 times I have done my western blotting, in which not all the protein in my gel is transferred efficiently to PVDF membrane and we use wet transfer method for blotting 100 volts for...

24 July 2014 3,818 9 View

Is anyone aware of names of proteins that show large bending movements that are functionally relevant?

I would like to get the names of a few proteins that show bending dynamics in solution. Are there any transport proteins that shown similar movements? Kindly post them here. Thanks in advance.

12 June 2014 6,727 7 View

What is the energy barrier from hinge movements (like bending and twisting) in proteins?

In two domain proteins there are movements possible along the hinge. Is anyone aware of the energy involved in this hinge movement?

12 June 2014 4,207 8 View

Why are we quantifying the gene of interest say erbb3 with the housekeeping gene? What is the concept behind it?

And can any one explain to me the delta Ct method that is used to quantify the gene of interest?

04 May 2014 8,959 14 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View