Can anyone give me a protocol for cell viability assay using propidium iodide by FACS?

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How can i use RFLP Method for these SNPs ARID5B rs4948496, DHFR rs1650723?

these SNPs do not have restriction enzymes is it possible to do RFLP

15 May 2024 7,974 1 View

I need a protocol for PI staining using Ethanol as a fixative. Online protocols are to do with kits and I have only PI powder. Can anyone share?

I need to do fluorescent microscopy using Propidium Iodide. I initially fixed my cells with 4% paraformaldehyde and saw red stain in Control cells. It turns out PFA is cell permeable. So if anyone...

27 February 2024 437 3 View

Statistical analysis on maternal health care?

maternal health care

25 February 2024 8,730 3 View

How we search a research paper ?

Maternal health care

25 February 2024 4,309 1 View

How to solve this error associated with WRF-Fire?

FIRE:crash: wrf: SUCCESS COMPLETE WRF. Fire has reached domain boundary. -------------- FATAL CALLED --------------- FATAL CALLED FROM FILE: LINE: 152 crash: wrf: SUCCESS COMPLETE WRF. Fire...

06 February 2024 6,541 2 View

Is there a reference genome for RNA sequencing in Tetrahymena?

Hi! I am doing total RNA sequencing with a Nanopore sequencer to look at gene expression in Tetrahymena when mitosis is inhibited vs. normal cell division. Does anyone know if there's a reference...

16 October 2023 5,700 0 View

How to use google search volume to get absolute search data?

09 March 2023 8,876 2 View

How to purify DNA random pool (or library) after solid phase DNA synthesis and deprotection from solid support?

We have synthesized random DNA library (containing N40 random region) for aptamer selection using solid phase DNA synthesis. But after deprotection and gel purification followed by desalting, we...

09 October 2022 5,100 4 View

To test degree of protein-protein interaction, should I co-transform bait and prey constructs or mate them?

Hi, I want to check interaction strength between my protein of interest and several candidate interactors using Y2H (TakaraBio, previously clontech). If I follow manufacturer's instructions which...

11 April 2022 7,840 4 View

What is the glassware to be used to etch over 60 degree Celsius?

Suitable glassware for etching mechanism using strong acids at elevated temperatures.

14 October 2021 8,708 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View