I am investigating the mechanism of action of a cationic peptide against MTB. I suspect one of the mechanisms is by the Fenton reaction. One way I would like to prove this is by use of thiourea and dipyridyl, as they have been used before in inhibiting the Fenton reaction.

Does anybody have an idea of what concentrations I should begin looking at against MTB?

Has anybody got any experience investigating and inhibiting Fenton reaction again against MTB?

I would also like to ask about the preparation of thiourea and dipyridyl. Should it be added to bacterial media, then autoclaved? Or should the compounds be added to the autoclaved media using filter sterilization?

Thanks