How to choose the best segmentation model using the area under the precision recall curve, IOU and Dice metrics?

I am using several U-Net variants for a medical image segmentation task. I get the following values for the performance measures including Dice, IOU, Area under receiver-operating characteristic...

27 December 2020 7,273 3 View

How to perform pixel-level mappings between different views of chest x rays?

I have a frontal and lateral chest x ray projection for a given patient as shown in the attached figures. I am thinking about performing pixel-level mappings across these views. What are the steps...

26 October 2020 7,309 3 View

Do we have better image synthesis methods other than GANs to synthesize medical images?

We have seen researchers using various types of GANs to synthesize medical data. However, it is hard to train the GANs and also the synthesized images are no good compared to the actual since...

20 July 2020 6,963 4 View

What quantitative measures to use to explain variance across datasets?

I have observed that the performance of a machine learning model trained on a larger dataset is not as convincing as the one trained on a smaller (different) dataset. What quantitative measures I...

16 February 2019 9,398 4 View

How to extract features from different layers of GoogLeNet in Matlab?

Commonly we extract features using: net = googlenet() %Extract features featureLayer = 'pool5-drop_7x7_s1'; #the last layer before softmax How to extract features from a different layer earlier in...

29 November 2017 3,753 4 View

What are the factors to be considered while performing Homology modeling of a protein ? also suggest some best tools for above ?

23 September 2017 8,567 7 View

What are the factors to be considered while performing Molecular docking analysis ? what should be mentioed in result interpretation part ?

factors / parameters for molecular docking result interpretation

01 August 2017 1,618 2 View

Open source database for TB Images?

Do we have a open-source standardized database of TB microscopic sputum smear images?

24 July 2016 5,251 5 View

Can anyone suggest an alternative website for archiving theoretical protein structures other than PMDB?

PMDB link is down for the last two months. I need help to archive and get a unique id for my homology modeled protein structure. Any suggestions?

04 May 2014 9,448 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

No title

02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View