Thanks.
This has really been quite frustrating. Every time I load my samples (20 to 45 ug per well), I see lines form in the resolving gel and they interrupt the run. The proteins just accumulate on that...
11 December 2019 7,721 10 View
I prepared M9 medium by autoclaving the M9 salts and glucose separately. Calcium chloride, magnesium sulfate, and trace minerals were dissolved in autoclaved water and sterilized by filtration....
05 June 2018 5,125 4 View
I am noticing that in thermostability assays call for heating the enzyme at the desired temp for a set time and then immediately placing in ice before carrying out the assay (as reported in...
02 March 2018 8,544 0 View
Any suggestions? Perhaps in NaOH and the neutralize to pH 7. I need the pH to be around 7 in the final solution.
01 February 2018 5,757 8 View
If I am using substrate concentration that is much lower than the reported Km of an enzyme, say for example 30x lower, is there a way to compensate for that? Can I just add an excess of enzyme...
31 December 2017 4,925 3 View
So, I'm doing an enzyme assay and there are two compounds in the assay, one a product and the other a cofactor, that absorb at the same wavelength. It makes it very difficult for me to detect...
31 December 2017 3,810 3 View
What kind of chemical agents can I use to oxidize NADPH in a reaction and stop an enzymatic reaction? Suggestions other than chemical agents are also welcome. Thanks!
31 December 2017 1,869 5 View
When you have an antibiotic resistance gene in a plasmid, how do ensure that the enzyme is constitutively expressed so that your selection always works?
10 November 2017 5,410 5 View
I am doing some enzyme assays with a non-native substrate of an enzyme. At a certain concentration, it seems to plateau (as if reaching Vmax) and the graph resembles MM kinetics but when I try...
07 August 2017 2,393 15 View
I've been thinking a lot about this question of late. I suppose most people determine kinetic parameters in terms of Km and Kcat to see how well their substrate is catalyzed by an enzyme. But if a...
05 June 2017 5,431 11 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...
20 July 2024 1,339 2 View
Hello researchers, I have sequenced the A549 cell line using RNA sequencing approach and then I have it against the human genome reference sequence. Would I expect a 100% mapping percent? I got...
20 July 2024 9,705 0 View
Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
18 July 2024 1,579 2 View
We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...
16 July 2024 118 2 View
I have attempted various methods, including homozygous gene disruption and CRISPR-Cas9, but have not achieved the desired results. It appears that the gene of interest is situated in a...
15 July 2024 3,977 1 View
is there any ways that i can find genome similarity of an organism without whole genome sequencing ,like using maths formulas or experimental progress ?
05 July 2024 4,070 3 View
