Thanks.
This has really been quite frustrating. Every time I load my samples (20 to 45 ug per well), I see lines form in the resolving gel and they interrupt the run. The proteins just accumulate on that...
11 December 2019 7,305 10 View
I prepared M9 medium by autoclaving the M9 salts and glucose separately. Calcium chloride, magnesium sulfate, and trace minerals were dissolved in autoclaved water and sterilized by filtration....
05 June 2018 4,688 4 View
I am noticing that in thermostability assays call for heating the enzyme at the desired temp for a set time and then immediately placing in ice before carrying out the assay (as reported in...
02 March 2018 8,339 0 View
Any suggestions? Perhaps in NaOH and the neutralize to pH 7. I need the pH to be around 7 in the final solution.
01 February 2018 5,332 8 View
If I am using substrate concentration that is much lower than the reported Km of an enzyme, say for example 30x lower, is there a way to compensate for that? Can I just add an excess of enzyme...
31 December 2017 4,579 3 View
So, I'm doing an enzyme assay and there are two compounds in the assay, one a product and the other a cofactor, that absorb at the same wavelength. It makes it very difficult for me to detect...
31 December 2017 3,510 3 View
What kind of chemical agents can I use to oxidize NADPH in a reaction and stop an enzymatic reaction? Suggestions other than chemical agents are also welcome. Thanks!
31 December 2017 1,601 5 View
When you have an antibiotic resistance gene in a plasmid, how do ensure that the enzyme is constitutively expressed so that your selection always works?
10 November 2017 5,123 5 View
I am doing some enzyme assays with a non-native substrate of an enzyme. At a certain concentration, it seems to plateau (as if reaching Vmax) and the graph resembles MM kinetics but when I try...
07 August 2017 2,109 15 View
I've been thinking a lot about this question of late. I suppose most people determine kinetic parameters in terms of Km and Kcat to see how well their substrate is catalyzed by an enzyme. But if a...
05 June 2017 4,984 11 View
We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...
13 February 2021 9,174 1 View
When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...
09 February 2021 5,423 1 View
I urgently need a map that shows the point locations of informal settlements in South Africa. I am currently working on a study that seeks to relate protests location to the location of informal...
02 February 2021 4,113 7 View
I am performing whole genome sequence for gram negative acinetobacter baumannii using two platforms illumina miswq and oxford nanopore. Thus i require a very high yield and high purity. I am using...
31 January 2021 355 2 View
I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...
29 January 2021 7,764 6 View
I have a list of gene names from deep sequencing from a constructed CRISPR knockout library. I was hoping to write a script to annotate all the gene names with a quick description of product...
27 January 2021 8,035 1 View
To be precise, I am looking for studies on informal labour that have taken on board Sanyal's category of the Need Economy and his 'Politics of Exclusion' in studying the political-economy of...
26 January 2021 4,219 3 View
Hi all, Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend...
26 January 2021 8,732 3 View
Hello, I'm working on primers from the publication: Okodo M, Okayama K, Teruya K, Sasagawa T. Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types. J Med Virol....
24 January 2021 7,636 3 View
Hi, I have raw data from [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array that I want to process using the Expresso function for Affymetrix microarrays. My samples include tumor...
22 January 2021 1,527 3 View
