How do you co-culture astrocytes and neurons?

I am planning to co-culture primary astrocytes with immortalized neurons GT1-7 (both from mice). What is the best way to do this?

05 November 2020 5,040 4 View

HOW CAN I DO AWAY WITH ERROR TERM HAVING ZERO DEGREE OF FREEDOM SO THAT TUKEY POST HOC TEST CAN BE PERFORMED.

04 May 2017 3,828 2 View

Can anyone point me to the best papers describing the format and use of 'User Stories' during requirements analysis/engineering?

Can anyone point me to the best papers describing the format and use of 'User Stories' during requirements analysis/engineering?

24 November 2015 4,448 3 View

What statistical analysis would be most appropriate for this (Forensic Entomology) data?

I have data from a 'crime scene' with species and number of individuals from both a control patch of soil and from a shallow grave. I am looking to see if there is a significant difference in the...

12 November 2015 6,962 2 View

What statistical analysis would be most appropriate for this (Forensic Entomology) data?

I have data from a 'crime scene' with species and number of individuals from both a control patch of soil and from a shallow grave. I am looking to see if there is a significant difference in the...

10 November 2015 4,228 4 View

Anyone familiar with abaqus Shell Elemet Stress Recovery?

07 May 2015 9,036 1 View

Is there a large animal model for pancreatic adenocarcinoma?

I am looking to use arterial caterterization for PDAC treatment.

23 December 2013 5,318 2 View

What software do you use to write and organize fieldnotes?

(Specifically for Mac software)

03 May 2013 7,170 19 View

How do I view the symbology for 4 band raster imagery in ArcMap? Is the alpha symbology understood as band 4 and does not appear in the TOC?

This is for NAIP imagery of California from 2009.

13 December 2012 3,287 2 View

In several situations which exploits the interrelationship between various granular layers, would three-way decisions be an effective method for GrC?

Prof. Yao, construction of granules into three regions may restrict other multi-level, multi-view representations associated with reasoning with granules. However, to solve a problem at hand...

01 January 1970 5,508 0 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View

Can I use a categorical covariate in a Generalized Linear Model in SPSS v.26?

Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...

14 February 2021 8,937 4 View

Mixed ANOVA or Multilevel modeling?

Hi everyone, I'm having some questions about choosing the right test for my experiment and I hope I can find help here! Here are the settings of the experiment: 1. participants are randomly...

14 February 2021 1,028 4 View

Defining mixed model with repeated measures data?

HI all, I have been working with daily diaries data ( 6 emotions of which 3 positive and 3 negative) were assessed for 30 days. These observations (1level data) are then nested within subjects...

13 February 2021 1,810 3 View

What non-parametric alternative can I use for a repeated-measures ANOVA for 2 groups and 2 timepoints?

Hello, I have a question regarding non-parametric alternatives for a RM-ANOVA. In my study I had two groups that were measured on two timepoints (pre-post measurements only). I was planning to...

11 February 2021 8,599 7 View