Is dynamic range related with stains or with a gel-based method and non-base approach?
Hello
I prefer to use the Coomassie Blue,when used as an infrared dye, may provide sensitive, post-electrophoretic in-gel protein detection that can rival even Sypro Ruby. Considering the central role of 2D gel electrophoresis in topdown proteomic analyses, a more cost effective alternative such as Coomassie Blue could prove an important tool in ongoing refinements of this important analytical technique.
Good Luck
Bastos
In simple words, is the confident relation between the low and high concentration that you can analyze in any experiment.
Regards
Thanks, Aniel.
So, when I am comparing two methods, is the highest value the best? Can I improve the dynamic range in 2-DE by enrichment and using Sypro instead Coomassie?
Hello
I prefer to use the Coomassie Blue,when used as an infrared dye, may provide sensitive, post-electrophoretic in-gel protein detection that can rival even Sypro Ruby. Considering the central role of 2D gel electrophoresis in topdown proteomic analyses, a more cost effective alternative such as Coomassie Blue could prove an important tool in ongoing refinements of this important analytical technique.
Good Luck
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