C.E.A. Bastos

9 Questions 7 Answers 0 Followers

Questions related from C.E.A. Bastos

How many times can I reuse gus satining solution?

Is there a more economical protocol to determine gus activity? In my protocol I use 10 mg X-Gluc/10 ml of work solution, can I use fewer X-Gluc? And how many this solution can be reused? Thanks.

05 September 2016 5,032 6 View

How can I calculate the growth relative coefficient (GRC) in plants?

I made an experiment with growth analyse and I want to know the contribuition of each parameter (SLA, LMF and NAR) to plant growth (RGR). So, I intend to calculate the the GRC for each parameter...

09 May 2016 6,388 2 View

Hello. Does someone have a protocol to observe tubulin in plant tissue by confocal microscopy?

I need a protocol to analyze tubulin in plant tissue. I want to visualize the structure of tubulin and I don´t know what tissue I have to use neither the protocol to prepare the samples to...

16 March 2016 4,497 2 View

How can I perform a secondary metabolism perfil of plants?

I am working with a native medicinal plant and I need a protocol to discover its essential compounds, but I have no idea what kind of compounds I will find.

21 May 2015 727 8 View

Does someone have a chromatogram for hemicelluloses (rhamnose, fucose, arabinose, xylose, mannose, galactose, and glucose)?

I need the chromatograms for hemicellulose in GC-MS. I'm using alditol-acetates protocol to derivatized plant cell wall and I need to check the peaks of polysaccharides rhamnose, fucose,...

28 April 2015 7,920 3 View

Does someone have a simple and economical polyacrylamide gel staining protocol for glycoproteins?

I intend to analyze plant glycoproteins and I would like to know a protocol to do it. Is it better use Pro-Q Emerald or PAS?

31 March 2015 5,179 5 View

What happens to the lignin content of plants, if the endogenous levels of salicylic acid increase?

Salicylic acid is biosynthetically related to lignin, so I need to understand what happens with secondary plant cell walls when salicylic acid is applied in plants.

20 February 2015 6,579 4 View

How can I alter dynamic range in shotgun proteome analyses?

I need to write a review about dynamic range in non-gel based proteomics approach, but I am not understanding the difference between dynamic range in gel-based and non-gel based approach.

08 February 2015 8,470 1 View

Can anyone explain to me what dynamic range in the context of proteomics means?

Is dynamic range related with stains or with a gel-based method and non-base approach?

07 February 2015 5,299 4 View