Can anyone explain me or join to me a reference about abclonal seamless cloning?

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Martin Chenal

Seamless cloning integrates PCR technologies into traditional, restriction-ligation cloning, making it a more versatile tool Abclonal is a brand like any other.

Here is a very helpful link about PCR cloning. This website also has many other user-friendly cloning protocols, which are perfect for neophytes: https://www.addgene.org/protocols/pcr-cloning/

To better understand the difference between traditional and seamless cloning : https://international.neb.com/applications/cloning-and-synthetic-biology/seamless-cloning.

Feel free to also check documentation about Gibson Assembly.

Hope it helped

Prof. Gheita

Dear Abire Sigbessia

Check these

https://www.cell.com/trends/biotechnology/fulltext/S0167-7799(05)00054-5

https://www.nature.com/articles/srep44648

https://international.neb.com/applications/cloning-and-synthetic-biology/seamless-cloning