I 'm expressing my protein and after inducing with IPTG the bacteria pellet turns to purple when I collected it, but the control didn't turn to pellet, it is white like usually, what can be the reason of the purple color?
I used pet16b vector to clone my gene of interest to further purify the protein .I added the fluorescent tag at the c-terminal for further microscopy. After cloning ,samples were sent to sequence...
15 July 2020 4,094 2 View
I used the pet16b containing an histagg to purify my protein of interest. Ni-NTA beads were used to purify the protein. after washing, the protein was still present when runned on the sds-gel...
19 May 2020 9,021 4 View
I'am new in seamless cloning, I don't know how it works. Can someone help or give a doc where I can learn about that? i'm talking precisely about abclonal seamless cloning.
08 January 2020 3,098 3 View
Do we need to place the insert in a specific site in his tagg vector for further protein purification or can we just use any restriction site present in the vector?
18 September 2019 6,933 3 View
i'm trying to clone my fragment of 255bp with nde1 and xho1 restrictions enzymes but still I don't get the fragment. i used 1:3 ratio,1:ratio,1:6 ratio but I always get only the recombinant...
30 April 2019 8,221 11 View
sometimes the protein still express even without adding iptg ; so what is the final role of IPTG?
06 April 2019 2,103 3 View
I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...
11 August 2024 4,793 1 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
SMEs are backbone of the country's white economy, most of them are II generation entrepreneurs and migrated from family owned business policies, and having sufficient higher educations for...
01 August 2024 5,562 2 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
Using SPSS, I made my edits in one bar chart — e.g., font type and size, hiding grid lines, and colours, namely: blue, orange, green, purple, and grey for 5 bars, respectively, etc. — and I saved...
29 July 2024 1,981 1 View
Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...
28 July 2024 6,515 1 View
Liu Zhaosheng. 2003. Triassic and Jurassic sporopollen assemblage from the Kuqa Depression,Tarim Basin of Xinjiang,NW China. Palaeontologia Sinica, New Series A,no. 14(Whole no. 190)....
28 July 2024 6,390 1 View
Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color
28 July 2024 286 0 View