I 'm expressing my protein and after inducing with IPTG the bacteria pellet turns to purple when I collected it, but the control didn't turn to pellet, it is white like usually, what can be the reason of the purple color?
I used pet16b vector to clone my gene of interest to further purify the protein .I added the fluorescent tag at the c-terminal for further microscopy. After cloning ,samples were sent to sequence...
15 July 2020 3,919 2 View
I used the pet16b containing an histagg to purify my protein of interest. Ni-NTA beads were used to purify the protein. after washing, the protein was still present when runned on the sds-gel...
19 May 2020 8,903 4 View
I'am new in seamless cloning, I don't know how it works. Can someone help or give a doc where I can learn about that? i'm talking precisely about abclonal seamless cloning.
08 January 2020 2,975 3 View
Do we need to place the insert in a specific site in his tagg vector for further protein purification or can we just use any restriction site present in the vector?
18 September 2019 6,816 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...
01 March 2021 6,187 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...
27 February 2021 947 7 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?
26 February 2021 3,487 3 View
Looking for method of extracting and analyzing vitamin D in foods by HPLC
24 February 2021 7,524 7 View
I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....
21 February 2021 8,609 1 View