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Questions related from Abire Sigbessia
I used pet16b vector to clone my gene of interest to further purify the protein .I added the fluorescent tag at the c-terminal for further microscopy. After cloning ,samples were sent to sequence...
16 July 2020 4,029 2 View
I used the pet16b containing an histagg to purify my protein of interest. Ni-NTA beads were used to purify the protein. after washing, the protein was still present when runned on the sds-gel...
20 May 2020 8,979 4 View
I'am new in seamless cloning, I don't know how it works. Can someone help or give a doc where I can learn about that? i'm talking precisely about abclonal seamless cloning.
09 January 2020 3,052 3 View
Do we need to place the insert in a specific site in his tagg vector for further protein purification or can we just use any restriction site present in the vector?
19 September 2019 6,893 3 View
i'm trying to clone my fragment of 255bp with nde1 and xho1 restrictions enzymes but still I don't get the fragment. i used 1:3 ratio,1:ratio,1:6 ratio but I always get only the recombinant...
01 May 2019 8,192 11 View
sometimes the protein still express even without adding iptg ; so what is the final role of IPTG?
07 April 2019 2,079 3 View
I 'm expressing my protein and after inducing with IPTG the bacteria pellet turns to purple when I collected it, but the control didn't turn to pellet, it is white like usually, what can be the...
26 March 2019 4,909 0 View
