Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is iron oxide (rust) a problem in DNA extractions?

Hello, I just started extracting DNA from some spiders, using 5-mm ball bearings to grind the specimens in a tissue mill. I had ordered the ball bearings on Amazon, and thought it was a good idea...

21 July 2020 2,271 6 View

Comparing two variables to one variable- is this possible in SPSS?

In a quantitative study, three variables-- I am comparing two variables (self-concept & well being) with another variable (intent to leave profession). I am predicting to see a positive...

05 May 2020 6,362 3 View

Is there a human gut microbiome database that shows normal variability as well as pathological vs normal ranges for specific microbes?

When a diagnostic lab furnishes normal ranges for certain microbes, is there any place to compare their ranges to what scientists are discovering is normal variability or adaptations? Is there a...

29 December 2019 4,771 2 View

Is it imperative in psychology research to state the null hypothesis?

I have had feedback from a reviewer that I need to restate my hypotheses as null hypotheses. My supervisor disagrees with this suggestion. Having done a bit of digging, I found Szucs &...

12 December 2019 1,447 4 View

How to fit data with binomial distribution?

Hello researchers, Which program or software works best to fit data with binomial distribution? Many thanks, Neena

15 November 2019 6,609 3 View

Stereochemistry Check for Peptides?

Hello researchers, Is PROCHECK the correct tool to check the stereochemical quality of small peptides? My peptide of interest: polypeptide with 16 alanine residues. Many thanks, Neena

28 October 2019 4,653 2 View

I have a GST tagged fusion protein in soluble phase will it have folded?

Normally this protein EIF4E is expressed untagged and expresses in inclusion bodies that are refolded through dialysis, but its very long winded and likes to precipitate during dialysis resulting...

11 June 2019 622 3 View

I am in the process of recruiting participants for my PhD using IPA and am now re-thinking the data collection design. Can you give me any advice?

28 April 2019 8,737 4 View

Approx. how much IL10 do activated T cells secrete after 48 hours in culture?

My students are culturing mixed mouse splenocytes in culture for 48 hours with anti-CD3/28. We are then going to analyse for the presence of Tregs and measure IL10 secretion by ELISA. In order to...

20 March 2019 5,857 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View