Luna Moon

3 Questions 2 Answers 0 Followers

Questions related from Luna Moon

Can anyone clarify the difference between IC50 and dose to me?

I guess I'm not understanding the concept well. If I treated cells (5000/well) with a range of doses from 6nM to 92nM (and this is an MTT assay and I've used Prism8), why is the IC50 only .9nM? I...

02 February 2020 8,127 2 View

Why should cancer cells be only 30-50% confluent when treating with drug after you've seeded them the previous day?

Is it because we are trying to target these cells before they have a chance to divide??? I guess I don't understand the logic behind this.

01 January 2020 7,589 3 View

What does it mean if an anti-cancer drug induces changes in cell signaling but not cell death?

I'm focusing on the Ras/Raf/MapK/Erk pathway and I'm expecting to see both when I treat with a MEK inhibitor in pancreatic cell cancer line. While I dramatically reduced levels of pERK, there's...

01 January 2020 4,436 4 View