I am facing problem in cloning. I am not getting colonies when tranforming my ligation into E. coli cells (DH5α strain). However efficiency of competent cells has also been checked using positive control where we are getting descent number of colonies. We have checked the elution also on agarose gel. It has desired band. I don't know what is going wrong.  Hopefully someone here can help me or get me on my way. 

Thank you in advance!

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