Elad Prinz

2 Questions 3 Answers 0 Followers

Questions related from Elad Prinz

Why do I measure unequal levels of expression for my reference gene in RT-PCR?

Hello, I'm researching gene expression of cells after some treatment, which cause apoptosis. when I compare 2 different population of cells (WT and crispr KO of some gene), I get the same Ct for...

04 February 2015 3,592 7 View

I see primer-dimers only in my qRT-PCR negative controls. Does it mean everything is OK?

Hello, I have just started with some qRT-PCR for my reasearch and Im doing a calibration of my reactions. With all the primers I checked(5 genes) I get an amplification of my NTCs and NRTs, in a...

07 January 2015 6,184 8 View