We planned to do gene expression study using disease suspected water sample.
If you want to store pure water for genetic studies, there is no difference for storage temperature. But, when the water contains RNA, the -80 C is the best.
For a water sample containing DNA, -20 is appropriate.
An important note about storage of Water samples containing nucleic acids is the way that you thaw the samples: You must be careful when you are thawing these samples.
For using the stored samples, you must thaw these samples by putting them, immediately, on ice. This way prevents samples to be shocked by sudden temperature change.
Good luck
Hi Karthika
if you planed RNA expression on your water samples, treat them as they were diluted RNA samples, the less temperature is the best (-80°c is reliable).
fred
Your samples are best stored at -80°C for gene expression studies.However,prolonged storage can increase risk of contamination and RNA recovery for other downstream processes such as sequencing,cloning,quality value etc
If you want to store pure water for genetic studies, there is no difference for storage temperature. But, when the water contains RNA, the -80 C is the best.
For a water sample containing DNA, -20 is appropriate.
An important note about storage of Water samples containing nucleic acids is the way that you thaw the samples: You must be careful when you are thawing these samples.
For using the stored samples, you must thaw these samples by putting them, immediately, on ice. This way prevents samples to be shocked by sudden temperature change.
Good luck
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