Which is the best mitochondrial genome annotation tool? Can anyone provide the list?

I have sequenced the mitochondrial genome of some moths species. I want to annotate and the sequence will submit in the NCBI.

19 November 2020 8,849 1 View

Lithium ion cell holder for Samsung 21700 - 50E cells?

Hello everyone, I have 5 Samsung 21700 - 50 E (NCM chemistry) cells. I want to do basic charge and discharge by connecting it in series. Does anyone know if there is a cell holder available...

05 October 2020 6,133 3 View

Which is the first complete mitochondrial genome sequenced lepidopteran Moth species?

I am preparing a research article about the complete mitogenome of moth. I want to include the details in my research article.

20 September 2020 8,151 3 View

Installation cost of a smart grid or Vehicle2grid ?

Hello, Does anyone know the cost to install Vehicle2grid service at home or commercial places ? I found a rough price as 5000$ in internet, but could anyone recommend a much cheaper solutions or...

07 July 2019 5,677 3 View

Is Oxidation of the PDMS surface necessary before silanizing it with APTES?

How imortant is it to oxidise the surface for silanization? I see some methods that use silnization buffer containing ethanol to activate the APTES and used it directly on PDMS without oxidation.

18 October 2018 5,806 3 View

Function in Scilab similar to Matlab xlswrite ?

Hi, Similar to Matlab xlswrite(filename,A), what is the function name in Scilab to write on a new excel file ? I tried searching, but couldn't find out. There was only write_csv...

25 April 2018 5,190 1 View

Scilab plot window not generated ?

Hi, When I try to plot in Scilab, no graphic window is generated (see pic). This is a simple plot between two columns of one matrix. Sometimes, I get error saying 'invalid index'. Does anyone know...

21 February 2018 3,548 3 View

Why capacitance value of 2RC battery model increases with temperature ?

18 September 2017 2,170 1 View

Why battery model fit goes bad with increase in C-rate ?

I am using 2RC Equivalent circuit model to fit discharge current pulses of LFP cell. I use 4 pulses (8C, 4C, 2C, 1C). The RMSE is good for 2C and 1C~5mV, but for 4C and 8C it seems to increase...

11 September 2017 6,960 3 View

How to fit current pulse data to impedance data

I have a set of discharge current pulse values (Voltage vs Time) as well as Impedance Spectroscopy data of Li-ion battery. How could I transfer these pulses to frequency domain and fit with other...

29 June 2017 5,590 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Which alginate is best for making gel for wound dressing application?

We are looking into utilizing alginate as a gel coating on an HDPE substrate for drug delivery. I would like to ask the community about their experience with alginate and which commercial sources...

15 February 2021 4,319 4 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Endothelial Functional Assays & Conditions?

Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...

05 February 2021 7,276 1 View

How to assing secondary structure to calculated peptide/protein having xyz file?

Hi, I have an optimized structure of some peptides and I would like to represent it as a secondary structure - cartoon. Ok, so server apps ask for pdb file (like Stride). I tried in different...

04 February 2021 517 3 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View