Challagandla Anil

5 Questions 14 Answers 0 Followers

Questions related from Challagandla Anil

Did anyone used advanced MEM to do transfections rather opti-MEM?

Dear Members, Kindly help me if anyone ever had used or having an idea about advanced MEM in cellular transfections. I am facing low transfection efficiency with lipofectamine 3000 when compared...

12 December 2019 7,775 3 View

How do we study long noncoding RNA being regulated by others?

Dear All, I am trying to over-express a lncRNA, but its expression is almost similar to the control, assuming everything is fine like promoter, cloning, transfection etc., why it isn't...

10 October 2018 4,783 1 View

How to filter/delete specific column values using R?

Dear All, I have a data frame (RNASeq), I want to filter a column (>=1.5 &

09 September 2018 5,002 3 View

Can anyone suggest a fatty acid culture media recipe for fungi?

I am trying to grow Colletotrichum truncatum in a synthetic media, where oleic acid as the sole carbon source. Thank you in advance.

06 June 2015 6,727 3 View

Can DMSO be added to protoplast?

Please can anyone suggest, weather DMSO is added (as a cryoprotectant) or not, to fungal protoplast. Since DMSO is a polar aprotic solvent I am scared of getting the protoplast membrane dissolved....

02 February 2015 1,803 8 View