Can you please help me to explain, why this attached SEM images show different effect of independent variable to dependent variables of same data?

Both Fig 1 and 2 describe aboveground biomass (AGB) and conducted SEM. However, the results were inconsistent. In fig.1, Nitrogen has significantly negative effect on AGB while in fig. 2, it was...

07 May 2020 6,280 3 View

Can we get 20 ensembles as output from NMR structures after energy minimization from YASARA ?

I am uploading 20 ensembles in the single PDB file, after energy minimization I just got one PDB structure. Is there any possibility to get 20 ensembles out?

23 March 2020 4,599 3 View

Is it economically feasible to produce biochar at industrial scale in developing economies?

In the context when majority of studies undertaken in various parts of worlds have reported beneficial effects of biochar to enhance agricultural productivity, governments in developing countries...

27 February 2020 8,426 11 View

Papers/codes for Threshold Panel Regression (PTR) using unbalanced panel data?

Hi All, While carrying out Panel Threshold regressions, most of the methods and statistical software seem to emphasize a balanced panel. For example, xthreg in STATA can only be used for balanced...

28 January 2020 9,683 4 View

Anyone here can tell me the difference between Positive cell sorting and Negative cell sorting using Flow cytometry (FACS)?

I am using negative sorting to isolate astrocyte but having some problem and i was thinking should i go to positive cell sorting? Does positive cell sorting has any superiority over negative cell...

11 December 2019 9,348 4 View

Is there any specific protocol (IHC) to work with the Phospho TAU in Rats brain?

HI, i am having difficulties with staining for Phospho TAU in KA-induced animal model. I am using free floating immuno histochemistry method. I am using AT-270, At-180, AT-100, AT-08 antibody...

14 January 2019 2,827 4 View

The effect of minus 300 micron particles on the workability of the concrete ?

it is experimentally proved that the minus 300 micron particles, if present in too little qty. will produce harsh concrete lacking cohesiveness and tending to seggregate. While much of its qty...

02 January 2019 8,989 2 View

Does subscriptions contributed significantly in impact factor?

if subscriptions significantly impacted on impact factor, it is worthless to be specific in subject specific journal. For example, better to target horticulture journal rather viticulture or pomology.

17 December 2018 3,916 3 View

Do phenological stages matter to count individual numbers of plant species of alpine grass for species richness?

I have counted numbers of individual of different species which have divided into eight different phenological states. Now I want to calculate diversity indices, should I need to add all stages of...

19 November 2017 803 10 View

Can anyone give idea to make competent cells from picha pastoris (KM71H) for electroporation transformation ?

13 July 2016 3,549 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View