i am dealing with callus generation of citrus limon as the leaves and shoots are very hard i have tried with cotyledons but the results are not up tp the mark...kindly help me out if any one can.
Please, read my following article: somatic embryogensis and plant let regeneration from nucellus tissues of local orange (citrus sinensis), Acta Agriculturae Slovinca,99,185-189,2012. you could download from my page on RG. I hope it will help you.
Which is the most actively dividing tissue in the nursery staged plant? You can select a few of them for explant inoculation. Sterilisation could be very challenging. Good luck.
sir,
thank you for the response but i have tried it with cotyledons and i want the callus on large scale which is not possible by using such a small explant which is not easily available.
can you suggest me some more ideas which i can implement?
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