Hi Shahabaldin

...just answered to your other SSF question. The FPU measurement is usually performed to have an activity close to your solid state reaction conditions...it is a little bit work to do, but I think it is ok to specify this once for your enzymes, to be sure from where you start. 

g/L ist the usual form for the sale of commercial enzymes, but you will need to have an acticity value to know exactly how much enzyme you ´will need to apply. Cellulase activity can be measured with different systems, maybe not every test gives you reliable information about acticvities under SSF conditions, specially if you want to compare different enzymes in different formulations. A solid enzyme will need to be dissolved to have better effects.

What kind of SSF do you perform and which MO do you use?

Thanks Dear Frank for your reply

Actually I want to use only one type of enzyme in sisimultaneous saccharification and fermentation, 

exactly this is my question, how much pure enzyme I have to add to my fermentation. my enzyme is cellulase pure adn in powder form. how can I be used? dry or liquied and which types of buffer needed?

Is it necessary to measure activity like FPU, Endoglucanase and b-Glucosidase? 

I usedNREL method to measure activity

Please find attached some papers of SSF for your better understanding

http://www.madep-sa.com/pdf/ssf.pdf

http://pierre.fkit.hr/hdki/cabeq/pdf/22_1_2008/Cabeq_%202008_01_06.pdf

http://pierre.fkit.hr/hdki/cabeq/pdf/29_2_2015/Cabeq%202015-02-web%20Sindhu.pdf

http://dspace.jdvu.ac.in/bitstream/123456789/19456/1/Acc.%20No.%20DC%2073.pdf

http://www.scielo.br/pdf/bjce/v22n4/26971.pdf