Can anybody suggest me appropriate tool for aligning and assembly of 574 sequences of 68 Mb data together?

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Stephen Tsoi

you can try to use galaxy https://usegalaxy.org/

Ratnesh Tripathi

Thankyou for your suggestion...

May I know if galaxy takes PacBio long read partial assembled data of 68Mb for aligning and assembly?

I am not familiar with PacBio NGS plateform and not sure galaxy has deveopled long read from PacBio. You can send your question to them. If not, Lasergene from DNAStar will do. They have input data for PacBio. Talk to them. GoodLuck!