You can find good information in:

De Minicis S, Kisseleva T, Francis H, Baroni GS, Benedetti A, Brenner D, Alvaro D, Alpini G, Marzioni M. Liver carcinogenesis: Rodent models of hepatocarcinoma and cholangiocarcinoma. Dig Liver Dis 2012 Nov 21. pii: S1590-8658(12)00406-9. doi: 10.1016/j.dld.2012.10.008.

is that possible to do in normal mouse, I mean to say swiss albino or Balb/c

Saad Hussain did refer to the appropriate literature. A few additional remarks ...as you do not want to contaminate anything with DEN, the i.p. route seems best. A single dose will do. the higher the dose, the faster you will find liver tumors but the higher dose will result in cytotoxic liver cell necrosis too. The exact protocol and dose will depend on the goal of your experiment. If you can accept toxic liver damage and some mortality your dose may even be close to LD50. For different mouse strains I would advise to have a small dose finding pilot study first. As DEN is hepatocarcinogenic in many species I am sure it is in any strain you want to use. Btw, do you have to use mice? Rats have the advantage that you can detect tumor prestages very early using histochemical markers which make the detection (and if necessary quantification) very easy.

a second thought... if you want to induce hepatocelluar tumors very fast the use of hatching eggs may be interesting. Inexpensive, no need for animal housing. We got hepatocelluar tumors in avian ( turkey) embryos within 24 days. I attach histology pics of these nodules.

Thank you harald >>>can you give some name of early detecting biomarker for hepatocellular carcinoma.....

in rodents, histology precedes everything else. You have a well defined development from preneoplastic foci to neoplastic nosules to malignant tumors.

For rats, placental form of glutathion-S-transferase (GSTP) is a very sensitive marker - and most probably the best. But even just looking for clear cell or basiphilc foci in standard H&E will show you preneoplasia.

Ok.. Sir thanks for your help, can you give me your email id , in near future is that any need i will be contact with you.

Please remember ,that DEN must be injected at day 14-15 otherwise as the hepatocytes stop proliferating, DEN alone protocol will give you no tumours. Please also refer to Michael Karin papers. What is your question behind doing this DEN experiment?