Can anybody suggest cheap and efficient company for whole genome sequencing of bacterial strains?

More Dipesh Dhakal's questions See All

Any one please give the idea about the NIST sp 800-22 test of image for check the randomness of bits in encrypted image ?

I have to find the randomness of encrypted image using NIST SP800-22 with(sequence 100 with each 1000000 bits or any number) but there are no such sufficient number bits then when running the...

05 June 2024 9,618 0 View

How to calculate the chi square test of grayscale image with randomly selected 2000 bits in python?

calculation of chi square test of gray scale image in python

26 May 2024 8,082 1 View

Can anyone provide me a single matlab code in which we can draw all the bifurcations, hope, flip and cusp bifurcation ?

Dear scientists, prof., researcher hope everyone is doing well. Can we draw all bifurcation in a single matlab code if yes then please provide me matlab code. Your cooperation is highly appreciated.

28 January 2024 2,095 3 View

When the data are provided in the median and IQR, How do we calculate proportion to perform pooled analysis?

The datasets are provided as medians and interquartile ranges. Can we perform a pooled analysis? How do we convert variables to get the events as (N)?

09 January 2024 5,348 2 View

Which plant life form is more vulnerable or resilient to the impacts of climate change? Herbs, shrubs or trees?

Some studies suggested that herbs are resilient to impacts of CC because they have shorter life span and are adaptive to CC. Other studies suggested that trees are more resilient because of the...

26 December 2023 9,560 1 View

How to prepare Yoghurt extract for estimation of antioxidant, flavonoid and phenolic compound?

I want to estimate the antioxidant, flavonoid and phenolic compounds of the fortified yogurt.

11 September 2023 1,158 2 View

How to conduct Hydrogen evolution reaction (HER) and Oxygen evolution reaction via the Gamry IFC1000-0745 software?

Hello, I have to test the water- splitting by the Hydrogen evolution reaction (HER) and Oxygen evolution reaction via the Gamry IFC1000-0745 software. Could you please help me how can I do the...

30 July 2023 2,729 4 View

What are the key measuring scales or criteria used to evaluate and quantify aspects of digital culture?

In the context of organizations, the concept of digital culture refers to the collective attitudes, beliefs, behaviors, and values that shape how technology is used, integrated, and perceived...

23 July 2023 7,985 0 View

I have to coat a CuO NPs via spin coating method, so how can i make a solution of CuO NPs for this process ?

I already made CuO NPs

18 July 2023 2,320 1 View

Can we do comparison between different type of Bifurcations. i.e. Hopf-bifurcation, Flip-bifurcation and Neimark-Sacker bifurcation ?

Can we draw all bifurcation in single MATLAB program. If yes, kindly provide me MATLAB program. Your cooperation would be highly appreciated.

23 June 2023 8,985 5 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

Daniela Pasanisi

Hi, I can suggest two Italian good companies:

www.bmr-genomics.it/ (I think only Italian site)

http://www.genomix4life.com/en/sequencing.html (also in EN)

Rongfeng li

I use ACGT, Inc. and I really like them.

Dipesh Dhakal

Can you please give me rough idea about the costs, so that I can decide the budgeting?