PEG coated Fe3O4 nanoparticles in aqueous NH3?

Hello, I synthesized PEG coated Fe3O4 nanoparticles by coprecipitation methods and I would like to ask whether they can be dispersed in aqueous NH3 since they have a PEG coating and theoretically...

06 February 2021 5,217 4 View

Is there such a function? Is it unique?

21 January 2021 8,962 13 View

What are the current developments in terms research and publications in anthropology in general and social anthropology in particular?

I am interested in examples of anthropological methods that have been applied across different disciplines, for exmple in mathematics education.

20 January 2021 3,090 3 View

How did the tests or exams get to Latin America, specifically the written tests?

I am looking for some bibliographic references that explain how tests or exams (measurement and assessment in education) came to Latin America, specifically written tests. I would like to know if...

13 January 2021 8,607 0 View

Is this true for all values c > 0?

25 December 2020 3,621 45 View

Can children from 1 year old to 15 years be given MODANA and Pfizer Anti-COVID-19 vaccine?

The clinical disease of COVID-19 has been low in children generally and vaccine has been develop.Any evidence of its safety,efficacy and risk for allergy in children? any exemption for children...

23 December 2020 7,177 12 View

Can large samples be stored at -20 degrees prior to RNA extraction?

As part of a Covid project we are sampling urban rodents and performing RNA extractions and serology work. We have too many samples to store at -80 and have opted to perform extractions the same...

15 December 2020 5,252 2 View

Substrate functionalization and antibody deposition for biosensor work?

Hello, I am a bio student working in a lab on his free time and I just started working with an engineering group on sensors. My questions are: 1)Functionalization steps and what are the best...

21 November 2020 7,996 2 View

Divergence detected in AMG solver for temperature and species , DEFINE_VR_RATE, Fluent ?

In 2D Model, Reaction in porous Zone, Error occurs on hooking UDF , DEFINE_VR_RATE in Fluent, .The error spikes just after running calculation after 1st iteration. Divergence detected in AMG...

16 November 2020 9,053 3 View

Why am I seeing RED fluorescence in control non transgenic plants?

Hi experts, I have cloned the DsRED gene into a plant vector under 35S promoter and used it for direct seed transformation of Chenopodium quinoa. When I checked the potential transformed...

24 October 2020 4,775 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View