I have got 22-25 colonies on my after transforming the ligation in DH5aplha. Should I screen all the colonies?

I have screened 10 colonies out of 22. But neither get insert release nor the PCR amplification for the desired gene. What should I do, should I further screen the colonies?

02 December 2020 3,225 3 View

Which bacteria can grow on 10x PBS buffer?

I have prepared PBS buffer and components for M9 medium and I store it in freeze, I do not use it if it is more than 2 weeks old. From time to time I check the sterility of these solutions and...

04 November 2020 9,427 6 View

Which model can be used to predict on below data?

I want to predict valuation using verbal SAS but there exists a strong nonlinearity between the two variables which makes unfit to use linear regression. Please see the attached scatter plot image...

05 August 2020 1,602 3 View

Which is the best model to predict student marks based on entrance exam result?

Hi, I have a dataset that contains student data that are nested based on different schools where more that one student belongs to a particular school. student_id school_id non_verbal...

29 July 2020 9,825 18 View

One step RT-PCR enzyme optimisation, can anyone help?

Helping others is the way we help help ourselves! In this case literally!! I work at a biotech company called Genaxxon Bioscience GmbH in Germany. We have an amazing one step RT-PCR enzyme which...

13 July 2020 7,395 2 View

Do Acetonitrile type (gradient grade or non gradient grade) affects the HPLC results?

Trying to develop the HILIC-RID method for sugar analyte but getting very different results between two-run of the same sample. Tried 60 minute washing between two-run. The mobile phase is 75:25...

11 June 2020 8,385 3 View

Why Pseudomonas fluorescens ATCC13525 does not grow on acetate?

I am trying to grow Pseudomonas fluorescens ATCC13525 on different carbon source except glucose in M9 medium but i cannot see them growing on acetate. I am not able to find more information about...

20 February 2020 2,469 6 View

Which funding agency in India will grant traveling allowance for international conference in finance ??

I get an opportunity to present my paper in international conference on June 2020. I am research scholar in management (finance department).Kindly let me know which funding agency will grant...

07 January 2020 7,040 1 View

Is raman peak at 515cm-1 normal for silicon wafer with 532 laser and 600 grating?

We installed new grating to your Renishaw inVia Raman microsscope. While routine calibration of the system, we observed the Si raman peak at 515cm-1 for 532 lase 600 grating and 519.9 cm-1 for 532...

02 December 2019 5,582 3 View

Why does my freshly thawed and seeded HeLa cells look different than usual?

I had stored HeLa cells in -80C freezer for over a month. When I thawed and seeded the cells today, I noticed that the cells looked significantly different than usual cells when floating in media....

25 November 2019 4,352 6 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

What is a diagnostic resistance in malaria?

For malaria research experts/Scientists I am experiencing a full explanation of the following concept: I have been looking at the biological threat of malaria to the control efforts, where the...

16 October 2020 1,563 4 View

What is the status of malaria vaccine?

Malaria is the highest killer disease in Africa, yet no vaccine has been developed against it. Any hope in sight?

05 October 2020 5,670 7 View

Are 12-week-old C57 mice resistant to cerebral malaria?

14 September 2020 460 4 View

Hydroxychloroquine Protonation in Blood and Cells - inconsistent information on claimed alkalinization inside targets, emphasis on Coronavirus?

Some researchers have claimed that Hydroxychloroquine (HCQ) can enter cells and make the environment more "alkaline" by soaking up protons. Many publications discuss only 2 nitrogen atoms that can...

08 August 2020 6,262 3 View

What is the mechanism of action of HCQ+Azi against covid 19 infection?

how H CQ+Azi acts against covid 19?

31 March 2020 4,043 9 View

What important factors are to be considered to met the objectives of malaria elimination program?

Countries like Ethiopia has developed malaria elimination program. But, I am not sure how this program is implemented .

10 November 2019 2,585 4 View

Please how can I find the primers and the restriction enzyme for my SNP: CLEC4C rs12310416?

I am working on the association of CLEC4C rs12310416 polymorphism with clinical malaria in children in South Western Nigeria, however, I need a publication that has worked on this before so I can...

08 September 2019 6,272 4 View

Where and how to get Research collaboration and PhD sandwich program?

I am currently carrying ou a study on Plasmodium faclciparum malaria and concomitant bacteria infection. I will need an expart to collaborate with especially in molecular epidemiology, and...

07 August 2019 9,877 1 View

Please under microscopy,can Malaria be differentiated into P. falciparum and P.ovale?

Thick film and Thin Film

07 August 2019 8,335 2 View