while doing column chromatography to extract glycosides with 1:2 ratio of ethyl acetate and petroleum ether I got elution's. based on color two of green and yellow elution's tested positive for sterols. is both are same or different sterols?
Jürgen Weippert
If the UV-Vis absorption (aka the complement of the perceived color) differs, you have different molecules in the mix. The reasons may be impurities, molecule sizes,terminations and others.
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