I'm looking for working antibodies against human skeletal muscle proteins for western blotting. Mainly I'm searching for SERCA I, dystrophin, alpha-actinin, actin, titin, nebulin as well as for the enzymes calpain I & II, but other suggestions are welcome too.
I have already troponin T, tropomyosin, desmin, vinculin, myosin, alpha-tubulin...
Thx! :)
The antibodies against Dystrophin from novocastra are perfect. They are used for diagnostic of DMD and Becker patients.
http://www.leicabiosystems.com/fr/ihc-ish/reactifs-novocastra/anticorps-primaires/details/product/dystrophin-antibodies/
I would advise you to use Labome (www.labome.com) to search for the antibodies. You can just search by the name of your gene (using gene symbols is recommended). If there is a link to "antibody review" (like in case of titin: http://www.labome.com/review/gene/human/titin-antibody.html) , this means that the antibodies are in VAD database and were used in indicated species for indicated appications, and this is described in a linked publication.
When you enter muscle in the search box of Everest Biotech you get all our antibodies to muscle proteins. Many would be fit for skeletal muscle and you can further select for human and for WB.
Thank you all for your answers! :)
I will check out all your suggestions :)
- Badges
- Science method