I am currently working on a project using Gammarus Pulex (Amphipod). Can anybody recommend at least three solvents that are not harmful to freshwater shrimps (Gammarus pulex), it should also be a good analytical solvent and to be as little complex as possible (i.e. not to contain too much noise).
Dimethyl Sulfoxide (DMSO), acetone, and methanol have been used. All are toxic to crustaceans at certain concentrations so it depends on how much is required for your purposes.
Can y use acetone ,DMSO,but all are toxic depends on the concentration that y will be use .
Dear Margaret Okundigie,
For the analysis of antioxidant enzymes activities in amphipods, I generally use acetone at 0.01% (final concentration). Be careful to add a solvent control in your experiment to compare the results.
Good luck
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