It lives in the dental calculus, the infected gum disease and purulent tonsillar crypts. T. tenax could be involved in the degradation of periodontal tissue by secretion of various substances such as alkaline phosphatase, and the fibronectin cathepsine as vaginalis. Trichomonas tenax fulfills the requisite of a parasite, causing damage to different mammalian cells and behaving similarly to Trichomonas vaginalis when in contact with target cells in vitro.It has no cysts and is transmitted directly from vegetative form.It lives in the dental calculus, the infected gum disease and purulent tonsillar crypts. T. tenax could be involved in the degradation of periodontal tissue by secretion of various substances such as alkaline phosphatase, and the fibronectin cathepsine as vaginalis. Trichomonas tenax fulfills the requisite of a parasite, causing damage to different mammalian cells and behaving similarly to Trichomonas vaginalis when in contact with target cells in vitro.It has no cysts and is transmitted directly from vegetative form.Trichomonas tenax can easily be detected by phase contrast microscopy on a fresh mounted smear of periodontal pocket biofilm infection.
Dear Anah
One of the simple and practical culture medium for detection and diagnosis of T.tenax and E.gingivalis in xenic culture is Hrs +S medium. (Horse serum,ringer and Starch).
For axenic cultivation TyI-s-33 is one of the best.
sincerely
Samples of calculus and plaque were taken from around the teeth during routine oral
examinations by sterile swab and immediately inoculated in to tubes of growth medium Dulbecco's Modified Eagle Medium (DMEM) and Minimum Essential Medium Eagle
(MEME) (2 ml of medium in each tube) Inoculated tubes were incubated at 35ºC, after 24 hours, 28 hours and 72 hours. A drop of culture media was removed from the tube
with a sterile Pasteur pipette in to glass microscope slide and covered with cover slip then examined by light microscope using a 40X objective. Results were represented as positive trophozoites or negative trophozoites.
PREPARATION OF DMEM CULTURE MEDIA
To prepare 1 liter of DMEM measure out 5% less than one liter of sterile distal H2O. Add 1 bottle of powdered media to room temp sterile distill H2O with gentle stirring (slowly to avoid clumping) until dissolve. Add 2.2 g of NaHCO3(Sodium Bicarbonate). The final pH of the media should be pH 7.4. Adjust pH of media by add 10 ml of HEPES Buffer. After pH has been adjusted, keep container closed until media is filtered. Sterilize immediately by using filter. Store media at 4°C in the dark for up to 12 months. When use media add 10 % (110 ml) Growth Media , 1 % (11 ml) P/S (Penicillin/Streptomycin) antibiotic ,and 1 %(11 ml) L-glutamine. Store media at 4°C in the dark for up to 1 month
PREPARATION OF MEME CULTURE MEDIA
To prepare 1 liter of MEME measure out 5% less than one liter of sterile distal H2O Add 1 bottle of powdered media to room temp sterile distill H2O with gentle stirring (slowly to avoid clumping) until dissolve. Add 2.2 g of NaHCO3. The final pH of the media should be pH 7.4. Adjust pH of media by add 10 ml of HEPES Buffer. After pH has been adjusted, keep container closed until media is filtered. Sterilize immediately by using filter. Store media at 4°C in the dark for up to 12 months. When use media add 10 % (110 ml) Growth Media, 1% (11 ml) L-glutamine and 0.10 g Na Pyruvate. 0.01mm non essential amino acids should be in media. Store media at 4°C in the dark for up to month.
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