The samples (mammals dry muscles and skin tissues) are about one hundered years old, an ammounts are about 0.05 g
Hi,
I cannot tell you anything about kits, but I can recommend these two protocols:
http://www.pnas.org/content/110/39/15758.short
http://genome.cshlp.org/content/early/2017/04/13/gr.219675.116.abstract
With them you can get the really short molecules from ancient samples. And they work fine for input amounts between 25-50 mg.
Best
Elena
I see, you don't take necessary care to details. Which are VERY important and may be critical when you deal with realy bad quality samples...
Nevertheless, thanks for reply.
you can use the manual method -- Phenol chloroform isoamyl alcohol .
best wishes
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