Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

How to determine the genetic potential of the genotype in terms of fruit size and weight?

In addition to genetic and environmental factors, fruit size is also influenced by crop load, rootstock, orchard planting system/tree training system, pollination, water and nutrient management,...

13 January 2021 9,309 4 View

Why am I getting 2 bands for Gibson Cloning Vector PCR?

Hi, I designed Gibson primers (2 primers for fragment and 2 primers for vector) using snapgene. Then I ran PCR separately for Fragment and Vector. Fragment DNA was found to be the correct sized...

11 January 2021 9,500 2 View

I am looking for a microbend optical sensor design...can anyone please provide me some idea about it??

I am working on a landslide model...i need an optical sensor to determine the amount of pore pressure and other values..i am thinking of using a microbend sensor. Can anyone please provide me with...

11 February 2021 5,911 2 View

How to download high quality DEM (resolution less than 5meter)?

I want high-quality DEM for our research purpose. High-quality DEM is used as a reference. I have generated the DEM through stereo images.

27 December 2020 8,614 15 View

How to plot a Slope gardient Map of Bathymetry?

In order to find out the area/location prone to submarine landslide, plot of slope gradient map of Bathymetry is very helpful. Guide me, how to plot of slope gradient map (in detail/steps) of...

05 November 2020 8,144 4 View

How can I apply ann, svm, rf, wof etc. machine learning models in landslide susceptible study?

For landslide susceptible mapping I have some influencing factors like, slope, elevation, distance from road etc.... but now how can I apply above mentioned models to get landslide susceptible map?

03 June 2020 5,372 3 View

How to seprate the grid cells of landsldie source areas using ArcGIS in batches?

The source areas of landslides are useful for accurately mapping the susceptibility of landsldies. However, an inventory of landslides always contains the source and deposit areas of the...

05 March 2020 5,582 3 View

What should be minimum coherence threshold for analysing DInSAR results for reliable Land movement detection?

We perform differential SAR Interferometry for analyzing the land movement. In case of Landslides the area generally suffers from decorrelation. So for the confidence in the result what minimum...

11 December 2019 2,360 3 View

Where i can find documented and published example of machine learning dataset for landslides problem ?

I am looking for documented and published example of machine learning data-set for landslides problem I need the data-set to be tested and published in IF journal. Example of the required paper...

08 September 2019 4,943 12 View

Where i can find documented and published example of machine learning dataset for landslides problem ?

I am looking for documented and published example of machine learning data-set for landslides problem I need the data-set to be tested and published in IF journal. Example of the required paper...

01 September 2019 9,572 13 View

Are you aware of any study about consequences of landslide restoration/stabilization for animals?

Dear all, I have been searching through the literature for examples of possible impacts on biological communities of interventions targeted at restoring / stabilizing landslides. I found just a...

16 August 2019 6,793 6 View

Are you aware of any study about consequences of landslide restoration/stabilization for animals?

Dear all, I have been searching through the literature for examples of possible impacts on biological communities of interventions targeted at restoring / stabilizing landslides. I found just a...

07 August 2019 8,923 7 View