JUN and ABY dye are not available in Biorad machine. Can any one tell me which dye (present in biorad machine) wavelength is similar with JUN n ABY ??

For covid19 diagnosis TaqPath kit is having ABY n JUN dye... But in our lab Biorad Cx96 machine is available where ABY n JUN dye are not available. How can we use the Biorad machine for covid19...

22 July 2020 1,978 2 View

How to set the number of samples, time steps and features in this data for an LSTM?

I have eight columns in my data with 3900 rows, column 1 is time (with 1-day interval) and the seven columns are the parameters related to car price and the ninth column represents sales of car...

18 April 2020 7,215 2 View

How regression problem can be solved with deep learning?

What will be a suitable model for solving the regression problem? Is there any hybrid algorithm or new model/framework exist to solve this problem in deep learning. How much deep learning is...

02 April 2020 7,640 3 View

Can anyone identify the two plant species

The plants were collected from Barak Valley Assam

24 May 2017 9,971 6 View

Sample making for ELISA

At first I treated interferon beta protein to the tumor bearing mouse then I collected the tumor and blood from the mouse. I already made the tumor sample with PBS and collected serum from blood....

01 January 1970 7,883 4 View

ELISA sample preparation

I want to check interferon beta protein concentration in mouse tissue sample like heart, liver, lung and kidney. Can anyone please help how can I make the sample from the tissue for ELISA?

01 January 1970 1,848 3 View

ELISA standard curve

when we calculate the concentration of unknown samples based on standard curve, which range of data can be used? Lowest and highest range?

01 January 1970 2,748 5 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View